CST, 16046S, Cleaved Caspase-6 (Asp179) (E8L9T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Casp6 (Asp179, 18 kD) cleaved. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Cleaved Caspase-6 (Asp179) (E8L9T) Rabbit Monoclonal Antibody (CST #16046) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Cleaved Caspase-6 (Asp179) (E8L9T) Rabbit Monoclonal Antibody recognizes endogenous levels of caspase-6 protein only when cleaved at Asp179. Non-specific bands were observed upon apoptosis at 25, 40, and 200 kDa in some cell lines. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp179 of human caspase-6 protein. Background Caspase-6 (Mch2) is one of the major executioner caspases functioning in cellular apoptotic processes (1,2). Upon apoptotic stimulation, initiator caspases such as caspase-9 are cleaved and activated (3). The activated upstream caspases further process downstream executioner caspases, such as caspase-3 and caspase-6, by cleaving them into large and small subunits, thereby initiating a caspase cascade leading to apoptosis (4,5). One of the major targets for caspase-6 is the membrane-associated protein lamin A (6). The cleavage of this protein causes cell membrane malfunction, membrane blebbing, and eventual cell death. Alternate Names Apoptotic protease Mch-2; CASP-6; CASP6; caspase 6; caspase 6, apoptosis-related cysteine peptidase; caspase 6, apoptosis-related cysteine protease; Caspase-6; Caspase-6 subunit p11; Caspase-6 subunit p18; MCH2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 18 Source/Isotype: Rabbit IgG