{"product_id":"cst-16046t","title":"CST,  16046T, Cleaved Caspase-6 (Asp179) (E8L9T) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Casp6 (Asp179, 18 kD) cleaved. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Cleaved Caspase-6 (Asp179) (E8L9T) Rabbit Monoclonal Antibody (CST #16046) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCleaved Caspase-6 (Asp179) (E8L9T) Rabbit Monoclonal Antibody recognizes endogenous levels of caspase-6 protein only when cleaved at Asp179. Non-specific bands were observed upon apoptosis at 25, 40, and 200 kDa in some cell lines.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp179 of human caspase-6 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCaspase-6 (Mch2) is one of the major executioner caspases functioning in cellular apoptotic processes (1,2). Upon apoptotic stimulation, initiator caspases such as caspase-9 are cleaved and activated (3). The activated upstream caspases further process downstream executioner caspases, such as caspase-3 and caspase-6, by cleaving them into large and small subunits, thereby initiating a caspase cascade leading to apoptosis (4,5). One of the major targets for caspase-6 is the membrane-associated protein lamin A (6). The cleavage of this protein causes cell membrane malfunction, membrane blebbing, and eventual cell death.\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nApoptotic protease Mch-2; CASP-6; CASP6; caspase 6; caspase 6, apoptosis-related cysteine peptidase; caspase 6, apoptosis-related cysteine protease; Caspase-6; Caspase-6 subunit p11; Caspase-6 subunit p18; MCH2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 18\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797628899497,"sku":"16046T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-16046t","provider":"Iright","version":"1.0","type":"link"}