CST, 16148SF, Bcl-11B (D6F1) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Bcl-11B. Validated for Western Blotting,Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, Bcl-11B (D6F1) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #16148) is ready to ship. Product Usage Information This product is the carrier free version of product #12120. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 12120 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Bcl-11B (D6F1) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total Bcl-11B protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu391 of human Bcl-11B protein. Background Bcl-11B (Ctip2) is a COUP-TF interacting protein that belongs to the C H type zinc finger protein family (1). Bcl-11B is highly expressed in the brain and is critical for the development of neurons, as well as other tissues and organs. Bcl-11B also plays an essential role in T cell lineage commitment and maintenance of T cell identity (1-3). Two isoforms of Bcl-11B are found to be encoded by the gene, possibly through exon-skipping (4). Bcl-11B is a transcription factor which binds to target genes through the 2 and 3 zinc-finger domains of exon 4 (3), while also interacting with various protein partners including COUP-TF proteins (1), the NuRD complex (5,6), HDAC1, HDAC2, and SUV39H1 (7). Research studies have shown that mutations and deletion of Bcl-11B contribute to the development of thymic lymphoma in mice and T cell acute lymphoblastic leukemia in humans, indicating a role as a tumor suppressor (4,8). Mechanistic studies have shown that Bcl-11B represses gene expression of the E3 ubiquitin ligase HDM2 in a p53-dependent manner (9). Alternate Names ATL1; ATL1-alpha; ATL1-beta; ATL1-delta; ATL1-gamma; B cell CLL/lymphoma 11B; B-cell CLL/lymphoma 11B; B-cell CLL/lymphoma 11B (zinc finger protein); B-cell CLL/lymphoma 11B/T-cell receptor delta constant region fusion protein; B-cell lymphoma/leukaemia 11B; B-cell lymphoma/leukemia 11B; BAF chromatin remodeling complex subunit BCL11B; BC11B; BCL-11B; BCL11B; BCL11B, BAF complex component; BCL11B/TRDC fusion; COUP-TF-interacting protein 2; CTIP-2; CTIP2; hRit1; hRIT1-alpha; IDDFSTA; IMD49; Radiation-induced tumor suppressor gene 1 protein; RIT1; zinc finger protein hRit1 alpha; ZNF856B Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 120-130 Source/Isotype: Rabbit IgG