{"product_id":"cst-16300t","title":"CST,  16300T, DGCR8 (E7A2J) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying DGCR8 mouse. Validated for Western Blotting,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, DGCR8 (E7A2J) Rabbit Monoclonal Antibody (CST #16300) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ®  Enzymatic Chromatin IP Kits.\nWestern Blotting: 1:1000\nChromatin IP: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Chromatin IP\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nDGCR8 (E7A2J) Rabbit Monoclonal Antibody recognizes endogenous levels of total DGCR8 protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of mouse DGCR8 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nDrosha was identified as a nuclear RNase III that catalyzes the initial step of microRNA (miRNA) processing (1). This enzyme processes the long primary transcript pri-miRNAs into stem-looped pre-miRNAs. Interference of Drosha results in the increase of pri-miRNAs and the decrease of pre-miRNAs (1). Drosha exists in a multiprotein complex called Microprocessor along with other components, such as DGCR8 (2). Drosha and DGCR8 are necessary for miRNA biogenesis (3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nD16H22S1742E; D16H22S788; D16H22S788E; D16Wi; D16Wis2; Dgcr8; DGCR8, microprocessor complex subunit; DiGeorge syndrome critical region 8 homolog; DiGeorge syndrome critical region gene 8; Gy1; Microprocessor complex subunit DGCR8; mir-1306; N41; OTTMUSP00000027994; OTTMUSP00000027995; Vo59c; Vo59c07\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 120\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797629358249,"sku":"16300T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-16300t","provider":"Iright","version":"1.0","type":"link"}