{"product_id":"cst-16433s","title":"CST,  16433S, Granzyme F Antibody","description":"Polyclonal Antibody for studying Granzyme F mouse. Validated for Western Blotting. Highly specific and rigorously validated in-house, Granzyme F Antibody (CST #16433) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nGranzyme F Antibody recognizes endogenous levels of total Granzyme F protein.\nSpecies Reactivity: Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln197 of mouse Granzyme F protein. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nGranzymes are a family of serine proteases expressed by cytotoxic T lymphocytes and natural killer (NK) cells and are key components of immune responses to pathogens and transformed cells (1). In mice, ten family members have been identified: A, B, C, D, E, F, G, K, M, and N (2). Granzymes are synthesized as zymogens and are processed into mature enzymes by cleavage of a leader sequence. They are released by exocytosis in lysosome-like granules containing perforin, a membrane pore-forming protein (3). Granzyme F induces a novel cell death pathway consisting of externalization of phosphatidylserine (PS), nuclear condensation, mitochondrial damage, cytochrome c release, single-stranded DNA nicking, and caspase inactivation (4). CTLs from mice with reduced expression of Granzyme F and C display diminished cytotoxicity (5). Â\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAKA granzyme G; C134; CCP; CCP4; Ctl; CTL serine protease 3; Ctla; Ctla-7; Ctla7; Cytotoxic cell protease 4; Cytotoxic serine protease 3; GRAF; Granzyme F; granzyme G; Gzmf; GZMH; MCSP; MCSP-3; MCSP3\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M\nSENSITIVITY: Endogenous\nMW (kDa): 30-40\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46801049288873,"sku":"16433S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-16433s","provider":"Iright","version":"1.0","type":"link"}