CST, 16473T, Gephyrin (E8T6V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying gephyrin. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Gephyrin (E8T6V) Rabbit Monoclonal Antibody (CST #16473) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Gephyrin (E8T6V) Rabbit Monoclonal Antibody recognizes endogenous levels of total gephyrin protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human gephyrin protein. Background The neurotransmitters GABA and glycine activate ligand-gated chloride channels and thus mediate fast synaptic inhibition. Gephyrin is a postsynaptic, scaffolding protein anchoring type A GABA and glycine receptors to the cytoskeleton. In addition to gephyrin's function of clustering synaptic neurotransmitter receptors, it plays an essential role in the biosynthesis of the molybdenum cofactor (MoCo). Molybdenum cofactor chelates and activates sulfite oxidase, an enzyme crucial for survival (1). GSK-3β and Erk1/2 phosphorylate gephyrin at residue Ser270 and Ser268, respectively. These post-translational modifications alter the clustering of gephyrin, affecting the amplitude and frequency of GABAergic inhibitory currents (2,3). Researchers are analyzing the role of abnormal gephyrin clustering and function in major neurological, neurodevelopmental, and psychiatric disorders (1). Abnormal accumulations of gephyrin are highly correlated with the neuropathologic diagnosis of Alzheimer's disease (4). Alternate Names Domain E; Domain G; GEPH; Gephyrin; GPH; GPHN; GPHRYN; HKPX1; KIAA1385; MOCODC; Molybdopterin adenylyltransferase; Molybdopterin molybdenumtransferase; MPT adenylyltransferase; MPT Mo-transferase Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 93 Source/Isotype: Rabbit IgG