{"product_id":"cst-16551t","title":"CST,  16551T, TCF1\/TCF7 (E6O1K) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying TCF7 mouse. Validated for WB,IHC,IF,IF,F,ChIP,C\u0026amp;R,C\u0026amp;T. Available in 2 sizes. Highly specific and rigorously validated in-house, TCF1\/TCF7 (E6O1K) Rabbit Monoclonal Antibody (CST #16551) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT\u0026amp;RUN dilution was determined using CUT\u0026amp;RUN Assay Kit # 86652 . The CUT\u0026amp;Tag dilution was determined using CUT\u0026amp;Tag Assay Kit # 77552 .\nWestern Blotting: 1:1000\nImmunohistochemistry (Paraffin): 1:200 - 1:800\nImmunofluorescence (Frozen): 1:400 - 1:800\nImmunofluorescence (Immunocytochemistry): 1:1600 - 1:3200\nFlow Cytometry (Fixed\/Permeabilized): 1:100 - 1:400\nChromatin IP: 1:50\nCUT\u0026amp;RUN: 1:50\nCUT\u0026amp;Tag: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 56853 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized), Chromatin IP, CUT\u0026amp;RUN, CUT\u0026amp;Tag\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nTCF1\/TCF7 (E6O1K) Rabbit Monoclonal Antibody detects endogenous levels of total TCF1\/TCF7 proteins. This antibody should detect all TCF1\/TCF7 isoforms, including those lacking the amino-terminal β-catenin binding domain. This antibody does not cross-react with LEF1, TCF3\/TCF7L1, or TCF4\/TCF7L2 protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant protein specific to mouse TCF1\/TCF7 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nLEF1 and TCF are members of the high mobility group (HMG) DNA-binding protein family of transcription factors that consists of the following: Lymphoid Enhancer Factor 1 (LEF1), T Cell Factor 1 (TCF1\/TCF7), TCF3\/TCF7L1, and TCF4\/TCF7L2 (1). LEF1 and TCF1\/TCF7 were originally identified as important factors that regulate early lymphoid development (2) and act downstream in Wnt signaling. LEF1 and TCF bind to Wnt response elements to provide docking sites for β-catenin, which translocates to the nucleus to promote the transcription of target genes upon activation of Wnt signaling (3). LEF1 and TCF are dynamically expressed during development and aberrant activation of the Wnt signaling pathway is involved in many types of cancers, including colon cancer (4,5). TCF1\/TCF7 has several isoforms due to alternative splicing and transcription from an alternative promoter. The isoforms generated by the alternative promoter do not contain the amino-terminal β-catenin binding domain and therefore may function in a dominant negative manner (6). TCF1\/TCF7 displays dynamic expression both in the total amount and the type of isoforms expressed in T cells during development and differentiation (7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAI465550; OTTMUSP00000005800; OTTMUSP00000005801; T cell factor-1; T-cell factor 1; T-cell-specific transcription factor 1; TCF-1; TCF-7; Tcf1; Tcf7; Transcription factor 7; transcription factor 7, T cell specific; transcription factor 7, T-cell specific\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 28-50\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797630701737,"sku":"16551T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-16551t","provider":"Iright","version":"1.0","type":"link"}