{"product_id":"cst-16563t","title":"CST,  16563T, FMC63 scFv (F2Q8G) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying CD19 Anti-Idiotype Ab. Validated for WB,IP,F,F. Available in 2 sizes. Highly specific and rigorously validated in-house, FMC63 scFv (F2Q8G) Rabbit Monoclonal Antibody (CST #16563) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nFlow Cytometry (Fixed\/Permeabilized): 1:400 - 1:1600\nFlow Cytometry (Live): 1:50 - 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Flow Cytometry (Fixed\/Permeabilized), Flow Cytometry (Live)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nFMC63 scFv (F2Q8G) Rabbit Monoclonal Antibody recognizes exogenously expressed levels of CARs containing an FMC63-derived scFv.\nSpecies Reactivity: All Species Expected\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with an scFv derived from a murine anti-CD19 monoclonal antibody, FMC63.\n\u003cb\u003eBackground\u003c\/b\u003e\nChimeric antigen receptor (CAR) T cell therapy utilizes synthetic receptors to redirect T cells to bind and eliminate tumor cells, bypassing traditional T cell receptor (TCR) signaling (1). The extracellular domain of conventional CARs consists of single-chain variable fragments (scFvs), which recognize and bind target antigens (1). CARs engineered to express the scFv of a murine anti-CD19 antibody, FMC63, have shown clinical success in targeting CD19-expressing malignant B cells for degradation. This clinical success has led to several FMC63-based CARs being approved by the U.S. Food and Drug Administration (FDA) for the treatment of B-cell leukemia and lymphoma (2,3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nB-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; B4; CD19; CD19 antigen; CD19 molecule; CVID3; Differentiation antigen CD19; MGC12802; T-cell surface antigen Leu-12\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: All\nSENSITIVITY: Transfected Only\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800194863273,"sku":"16563T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-16563t","provider":"Iright","version":"1.0","type":"link"}