CST, 16662SF, Phospho-EphA2 (Ser897) (D9A1) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying EphA2 (Ser897) phosphate. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, Phospho-EphA2 (Ser897) (D9A1) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #16662) is ready to ship. Product Usage Information This product is the carrier free version of product #6347. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 6347 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Phospho-EphA2 (Ser897) (D9A1) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of EphA2 protein only when phosphorylated at Ser897. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser897 of human EphA2 protein. Background The Eph receptors are the largest known family of receptor tyrosine kinases (RTKs). They can be divided into two groups based on sequence similarity and on their preference for a subset of ligands: EphA receptors bind to a glycosylphosphatidylinositol-anchored ephrin A ligand; EphB receptors bind to ephrin B proteins that have a transmembrane and cytoplasmic domain (1,2). Research studies have shown that Eph receptors and ligands may be involved in many diseases including cancer (3). Both ephrin A and B ligands have dual functions. As RTK ligands, ephrins stimulate the kinase activity of Eph receptors and activate signaling pathways in receptor-expressing cells. The ephrin extracellular domain is sufficient for this function as long as it is clustered (4). The second function of ephrins has been described as "reverse signaling", whereby the cytoplasmic domain becomes tyrosine phosphorylated, allowing interactions with other proteins that may activate signaling pathways in the ligand-expressing cells (5). Various stimuli can induce tyrosine phosphorylation of ephrin B, including binding to EphB receptors, activation of Src kinase, and stimulation by PDGF and FGF (6). Tyr324 and Tyr327 have been identified as major phosphorylation sites of ephrin B1 (7). It has been demonstrated that ligand-independent promotion of cell migration by EphA2 overexpression requires phosphorylation of EphA2 at Ser897 by Akt. On the other hand, stimulation of EphA2 by its ligand Ephrin-A1 negates Akt activation by growth factors and causes EphA2 dephosphorylation at Ser897 (8). Alternate Names ARCC2; CTPA; CTPP1; CTRCT6; ECK; EPH receptor A2; EPHA2; ephrin receptor EphA2; Ephrin type-A receptor 2; Epithelial cell kinase; epithelial cell receptor protein tyrosine kinase; protein tyrosine kinase; soluble EPHA2 variant 1; Tyrosine-protein kinase receptor ECK Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 125 Source/Isotype: Rabbit IgG