{"product_id":"cst-16737s","title":"CST,  16737S, Phospho-CaMKK2 (Ser495) Antibody","description":"Polyclonal Antibody for studying CaMKK2 (Ser495) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-CaMKK2 (Ser495) Antibody (CST #16737) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-CaMKK2 (Ser495) Antibody recognizes endogenous levels of CaMKK2 protein only when phosphorylated at Ser495. Bands of unknown origin are observed at 42 kDa and 25 kDa.\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser495 of human CaMKK2 protein. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nCalcium\/Calmodulin-dependent Protein Kinase Kinase 2 (CaMKK2) is a member of the CaMK family that contains a central Ser\/Thr kinase domain followed by a regulatory domain consisting of overlapping autoinhibitory and CaM-binding regions (1). CaMKK2 can be distinguished from other CaMK family members by the presence of a unique Pro\/Arg\/Gly-rich insert following the ATP-binding domain (2). CaMKK2 phosphorylates CaMKI at Thr177 and CaMKIV at Thr200 (3). CaMKK2 also phosphorylates AMPKα in response to calcium (4). CaMKK2 has been implicated in long-term memory formation (5) and adipocyte development (6). CaMKK2 is phosphorylated at Ser511 by death-associated protein kinase (DAPK) in a signaling cascade thought to be involved in neuronal death (7). Multiple CaMKK2 phosphorylation sites have been identified, including Ser495, and C-terminal phosphorylation of the CaMKK2 autoinhibitory region may regulate its function and localization (8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\ncalcium\/calmodulin dependent protein kinase kinase 2; calcium\/calmodulin-dependent protein kinase beta; Calcium\/calmodulin-dependent protein kinase kinase 2; calcium\/calmodulin-dependent protein kinase kinase 2, beta; Calcium\/calmodulin-dependent protein kinase kinase beta; CaM-kinase kinase 2; CaM-kinase kinase beta; CaM-KK 2; CaM-KK beta; CAMKK; CaMKK 2; CaMKK beta; CAMKK beta protein; CAMKK2; CAMKKB; KIAA0787; KKCC2; MGC15254\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nMW (kDa): 68, 70\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46797631062185,"sku":"16737S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-16737s","provider":"Iright","version":"1.0","type":"link"}