CST, 16836SF, Thy1/CD90 (D3V8A) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying THY1. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, Thy1/CD90 (D3V8A) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #16836) is ready to ship. Product Usage Information This product is the carrier-free version of product #13801. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 13801 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Thy1/CD90 (D3V8A) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total Thy1/CD90 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val62 of human Thy1/CD90 protein. Background The Thy1/CD90 cell surface antigen is a GPI-anchored, developmentally regulated protein involved in signaling cascades that mediate neurite outgrowth, T cell activation, tumor suppression, apoptosis, and fibrosis (1). Thy1/CD90 is highly expressed on the surface of adult neurons and is thought to play a role in modulating adhesive and migratory events, such as neurite extension (1,2). Decreased Thy1/CD90 mRNA and protein expression is associated with the development of epithelial ovarian cancer, suggesting a role as a putative tumor suppressor gene of human ovarian cancer (3,4). Research studies indicate that Thy1/CD90 knockout mice have impaired cutaneous immune responses and abnormal retinal development (5,6). Thy1/CD90 is epigenetically regulated or deregulated in some disease states, such as pulmonary fibrosis. The potentially reversible hypermethylation of the Thy1/CD90 promoter offers the possibility of novel therapeutic options in this disease (7). Alternate Names CD90; CDw90; FLJ33325; Thy-1 antigen; Thy-1 cell surface antigen; Thy-1 membrane glycoprotein; Thy-1 T-cell antigen; THY1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 22 Source/Isotype: Rabbit IgG