CST, 16864SF, Nucleolin (D4C7O) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Nucleolin. Validated for WB,IHC,IF,F. Highly specific and rigorously validated in-house, Nucleolin (D4C7O) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #16864) is ready to ship. Product Usage Information This product is the carrier free version of product #14574. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 14574 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Nucleolin (D4C7O) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total nucleolin protein. Species reactivity for IHC-P is human only. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly53 of human nucleolin protein. Background Nucleolin is a multi-functional protein that is one of the major components of the nucleoli (1). Nucleolin plays an essential role in various steps of ribosome biogenesis including rRNA synthesis, processing of pre-rRNA, pre-ribosomal RNA assembly, and transport of ribosomal proteins out of the nucleus (1-3). While the main function of nucleolin is ribosome biogenesis, it plays an important role in various other nuclear activities. Down regulation of nucleolin leads to increased expression of p53, defects in genome duplication, and a delay at prometaphase during mitosis leading to cell cycle arrest (4-6). In addition, nucleolin has been found in a complex with Rad51 and may participate in DNA repair by homologous recombination (7). Nucleolin binds to the catalytic subunit of the human telomerase reverse transcriptase, hTERT, and is thought to be involved in telomere maintenance (8). Nucleolin also possesses histone chaperone activity and is able to enhance the chromatin remodeling efficiency of SWItch/Sucrose Non Fermentable (SWI/SNF) and ATP-dependent chromatin-assembly factor (ACF), remove histone H2A-H2B dimers from nucleosomes, and facilitate the passage of RNA polymerase through chromatin (9). Alternate Names C23; FLJ45706; FLJ59041; NCL; Nsr1; NUCL; Nucleolin; Protein C23 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 100 Source/Isotype: Rabbit IgG