{"product_id":"cst-16975sf","title":"CST,  16975SF, Phospho-CSF-1R\/M-CSF-R (Tyr699) (D10B11) Rabbit Monoclonal Antibody (BSA and Azide Free)","description":"Monoclonal Antibody for studying CSF1R (Tyr699) phosphate. Validated for Western Blotting,ELISA+. Highly specific and rigorously validated in-house, Phospho-CSF-1R\/M-CSF-R (Tyr699) (D10B11) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #16975) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nThis product is the carrier free version of product #12251. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT\u0026amp;RUN or CUT\u0026amp;Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions\/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.\n\u003cb\u003eFormulation\u003c\/b\u003e\nSupplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 12251\n\u003cb\u003eStorage\u003c\/b\u003e\nStore at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze\/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-CSF-1R\/M-CSF-R (Tyr699) (D10B11) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of CSF-1R\/M-CSF-R only when phosphorylated at Tyr699. This antibody may cross-react with other activated tyrosine kinases including PDGF receptors and EGFR.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr699 of human CSF-1R\/M-CSF-R protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nMacrophage-colony stimulating factor (M-CSF, CSF-1) receptor is an integral membrane tyrosine kinase encoded by the proto-oncogene. M-CSF receptor is expressed in monocytes (macrophages and their progenitors) and drives growth and development of this blood cell lineage (1-3). Binding of M-CSF to its receptor induces receptor dimerization, activation, and autophosphorylation of cytoplasmic tyrosine residues used as docking sites for SH2-containing signaling proteins (4). There are at least five major tyrosine autophosphorylation sites. Tyr723 (Tyr721 in mouse) is located in the kinase insert (KI) region. Phosphorylated Tyr723 binds the p85 subunit of PI3 kinase as well as PLCγ2 (5). Phosphorylation of Tyr809 provides a docking site for Shc (5). Overactivation of this receptor can lead to a malignant phenotype in various cell systems (6). The activated M-CSF receptor has been shown to be a predictor of poor outcome in advanced epithelial ovarian carcinoma (7) and breast cancer (8). Phosphorylation of M-CSF receptor on Tyr669 was identified at Cell Signaling Technology (CST) using PhosphoScan , a CST LC-MS\/MS platform for phosphorylation site discovery, as well as in another publication (10). Autophosphorylation at Tyr699 in the kinase insert (KI) domain appears to provide a binding site for the Grb2 adaptor protein (9).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nBANDDOS; C-FMS; CD115; CD115 antigen; colony stimulating factor 1 receptor; CSF-1 receptor; CSF-1-R; CSF-1R; CSF1R; CSFR; FIM2; FMS; FMS proto-oncogene; HDLS; M-CSF-R; macrophage colony stimulating factor I receptor; Macrophage colony-stimulating factor 1 receptor; McDonough feline sarcoma viral (v-fms) oncogene homolog; Proto-oncogene c-Fms\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 175\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797631881385,"sku":"16975SF","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-16975sf","provider":"Iright","version":"1.0","type":"link"}