{"product_id":"cst-16984t","title":"CST,  16984T, CAR (D3W3G) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying CAR. Validated for WB,IP,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, CAR (D3W3G) Rabbit Monoclonal Antibody (CST #16984) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\nImmunohistochemistry (Paraffin): 1:500\nImmunofluorescence (Immunocytochemistry): 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 74159 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCAR (D3W3G) Rabbit Monoclonal Antibody recognizes endogenous levels of total CAR protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg328 of human CAR protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe coxsackie virus and adenovirus receptor (CXADR, CAR) is a highly conserved, single-transmembrane glycoprotein and the primary receptor to mediate cellular attachment and infection of coxsackie B viruses and most adenoviruses (1,2). The CAR protein contains a pair of Ig-like domains within the amino-terminal extracellular domain and a carboxyl-terminal PDZ motif (1). Research studies indicate that CAR is a tight junction protein that associates with the ZO-1 scaffold protein and promotes both cell adhesion and restriction of solute and ion movement between cells (2). Endogenous CAR is targeted to the basolateral plasma membrane by a tyrosine-based basolateral sorting signal and clathrin adaptors AP-1A and AP-1B (3). CAR binds junctional adhesion molecule L (JAML) on epithelial cells and neutrophils where it activates PI3K and downstream MAPK kinases to stimulate epithelial γδ T cell proliferation and increase production of TNFα and keratinocyte growth factor (4-6). As a result, the CAR protein plays a potentially critical role in adenoviral gene therapy, immunity, wound repair, inflammation, and cancer therapy (4-6). Additional studies demonstrate that CAR is essential in regulating squamous carcinoma cell growth (7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n46 kD coxsackievirus and adenovirus receptor (CAR) protein; CAR; CAR4\/6; coxsackie virus and adenovirus receptor; coxsackie virus B receptor; Coxsackievirus and adenovirus receptor; Coxsackievirus B-adenovirus receptor; CVB3 binding protein; CVB3-binding protein; CXADR; CXADR Ig-like cell adhesion molecule; CXAR; hCAR; HCVADR\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 45-55\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797631946921,"sku":"16984T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-16984t","provider":"Iright","version":"1.0","type":"link"}