CST, 16990T, PCGF2 (F9G6A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PCGF2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, PCGF2 (F9G6A) Rabbit Monoclonal Antibody (CST #16990) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity PCGF2 (F9G6A) Rabbit Monoclonal Antibody recognizes endogenous levels of total PCGF2 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly140 of human PCGF2 protein. Background Polycomb group RING finger homologs (PCGF1-6) are essential proteins for the assembly of the polycomb repressive complex 1 (PRC1) group of complexes. RING1A/B and a unique PCGF protein make up the catalytic core of PRC1 to ubiquitinate histone H2A. Differences in binding specificities give PRC1 complexes unique functions, depending on which PCGF is included (1-3). Polycomb group RING finger protein 2 (PCGF2), also known as Mel-18, is widely expressed in human tissues and primarily functions as a transcriptional repressor. This protein is broadly expressed in human tissues and has been implicated in numerous regulatory pathways that include cell proliferation and senescence, X chromosome inactivation, embryogenesis, hematopoiesis, and tumor suppression (4-10). Furthermore, this protein is known to harbor posttranslational modifications, including phosphorylation and ubiquitination, and is also reported to dimerize (11,12). Alternate Names DNA-binding protein Mel-18; MEL-18; MEL18; MGC10545; PCGF2; polycomb group ring finger 2; Polycomb group RING finger protein 2; RING finger protein 110; RNF110; TPFS; Zinc finger protein 144; ZNF144 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 50, 60 Source/Isotype: Rabbit IgG