CST, 17055S, VAMP5 (F9Y8C) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying VAMP5. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, VAMP5 (F9Y8C) Rabbit Monoclonal Antibody (CST #17055) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity VAMP5 (F9Y8C) Rabbit Monoclonal Antibody recognizes endogenous levels of total VAMP5 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human VAMP5 protein. Background Vesicle-associated membrane protein 5 (VAMP5), also known as myobrevin, is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) protein family, preferentially expressed in skeletal muscle and heart, with increased expression during myogenesis (1). While full functionality of VAMP5 is unknown, it has been shown to mediate exosome release through its interaction with SNAP47, syntaxin1, or syntaxin4 (2). VAMP5 has also been shown to promote Fcγ receptor-mediated phagocytosis and may play a role in regulating macrophage phagosome maturation (3). Plasma levels of VAMP5 are downregulated in Alzheimer's disease (AD), identifying the protein as a potential blood biomarker for AD when paired with other key differentially expressed proteins (4). Alternate Names Myobrevin; VAMP-5; VAMP5; vesicle associated membrane protein 5; Vesicle-associated membrane protein 5; vesicle-associated membrane protein 5 (myobrevin) Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 13 Source/Isotype: Rabbit IgG