CST, 17134T, PQBP1 (E5N7J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PQBP1. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, PQBP1 (E5N7J) Rabbit Monoclonal Antibody (CST #17134) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity PQBP1 (E5N7J) Rabbit Monoclonal Antibody recognizes endogenous levels of total PQBP1 protein. This antibody cross-reacts with a 50 kDa protein of unknown identity in mouse models by western blot. Mouse species reactivity is western blot only. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PQBP1 protein. Background Polyglutamine binding protein 1 (PQBP1) is an intrinsically disordered protein that is broadly expressed and evolutionarily conserved (1-3). PQBP1 has primarily been detected in the nucleus, though it has also been observed in the cytoplasm of mouse microglia (4,5). In the nucleus, PQBP1 is known to interact with mRNA splicing and RNA binding proteins, as well as the polyglutamine protein ataxin-1, and plays roles in both transcription and translation (4, 6-8). Mutations leading to loss of function of PQBP1 are associated with Golabi-Ito-Hall syndrome and Renpenning's syndrome, hallmarks of which include microcephaly and intellectual disability (9-11). Recently, PQBP1 has been reported to interact with tau protein, mediating activation of the cGAS-STING pathway in microglia (5). In the context of HIV-1 infection, PQBP1 also activates the cGAS-STING pathway by binding to reverse-transcribed HIV-1 DNA and cGAS, resulting in an innate immune response driven by IRF-3 (12). Alternate Names 38 kDa nuclear protein containing a WW domain; MRX2; MRX55; MRXS3; MRXS8; NPW38; nuclear protein containing WW domain 38 kD; polyglutamine binding protein 1; Polyglutamine tract-binding protein 1; Polyglutamine-binding protein 1; PQBP-1; PQBP1; RENS1; SHS Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 33 Source/Isotype: Rabbit IgG