CST, 17334S, CYP17A1 Antibody

Polyclonal Antibody for studying CYP17A1. Validated for Western Blotting. Highly specific and rigorously validated in-house, CYP17A1 Antibody (CST #17334) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CYP17A1 Antibody recognizes endogenous levels of total CYP17A1 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu473 of human CYP17A1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background CYP17A1, also known as cytochrome P450C17, is a steroidogenic enzyme belonging to the P450 cytochrome superfamily of monooxygenases (1, 2). In humans, CYP17A1 expression is abundantly expressed in the adrenal cortex, where it plays a central role in the androgen synthesis pathway (2). CYP17A1 is the primary target of abiraterone, a synthetic steroid used in the treatment of castration-resistant prostate cancer (CRPC) (3, 4). Abiraterone is converted to the more active form D4A, which antagonizes androgen receptor signaling by inhibiting CYP17A1 and other steroidogenic enzymes (3, 4). This suppresses the synthesis of 5α-dihydrotestosterone (DHT), which is a driver of castration-resistant prostate cancer cell growth (3, 4). Alternate Names 17-alpha-hydroxyprogesterone aldolase; CP17A; CPT7; CYP17; CYP17A1; CYPXVII; Cytochrome P450 17A1; cytochrome P450 family 17 subfamily A member 1; cytochrome p450 XVIIA1; Cytochrome P450-C17; cytochrome P450, family 17, subfamily A, polypeptide 1; cytochrome P450, subfamily XVII (steroid 17-alpha-hydroxylase), adrenal hyperplasia; Cytochrome P450c17; P450C17; S17AH; Steroid 17-alpha-hydroxylase/17,20 lyase; Steroid 17-alpha-monooxygenase Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 55 SOURCE: Rabbit