CST, 17402S, Nectin-4/PVRL4 Antibody

Polyclonal Antibody for studying Nectin 4. Validated for Western Blotting. Highly specific and rigorously validated in-house, Nectin-4/PVRL4 Antibody (CST #17402) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Nectin-4/PVRL4 Antibody recognizes endogenous levels of total Nectin-4/PVRL4 protein. Based upon sequence alignment, this antibody is not predicted to cross-react with PVR, PVRL1, PVRL2, and PVRL3 proteins. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Nectin-4/PVRL4 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Nectin-4/poliovirus receptor-related 4 (PVRL4) is a type-I transmembrane glycoprotein that belongs to the immunoglobulin superfamily and promotes cell-cell adhesion by serving as a major component of adherens junctions (1-3). The extracellular domain of Nectin-4, which contains an Ig variable-like domain (V) and two Ig constant-like domains (C), mediates binding to the measles virus (4) and to neighboring cells through heterophilic interactions with Nectin-1 (5,6). Unlike other nectin family members, which are widely expressed in adult tissues, Nectin-4 expression in humans is largely restricted to the placenta (7). Research studies have demonstrated that Nectin-4 is overexpressed in a variety of human solid tumors of the pancreas (8), breast (9,10), lung (11), and ovary (12). Due to its restricted expression pattern in normal human tissues, Nectin-4 may serve as a novel diagnostic and therapeutic target for a variety of human tumors. Alternate Names EDSS1; Ig superfamily receptor LNIR; LNIR; NECT4; nectin 4; Nectin cell adhesion molecule 4; Nectin-4; NECTIN4; poliovirus receptor-related 4; Poliovirus receptor-related protein 4; Processed poliovirus receptor-related protein 4; PRR4; PVRL4 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 62, 75 SOURCE: Rabbit