CST, 17433S, CMTM4 Antibody

Polyclonal Antibody for studying CMTM4. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, CMTM4 Antibody (CST #17433) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity CMTM4 Antibody recognizes endogenous levels of total CMTM4 protein. This antibody does not cross-react with CMTM6 protein. Species Reactivity: Human, Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu202 of human CMTM4 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background CKLF-like MARVEL transmembrane domain-containing protein 4 (CMTM4) is a member of the chemokine-like factor (CKLF)-like MARVEL transmembrane domain-containing family (1). CMTM4 acts as a tumor suppressor in various malignancies, and regulates cell growth and transition through the cell cycle in HeLa cells (1-4). CMTM4Â plays an important role in angiogenesis, enabling internalization of membrane-bound vascular endothelial cadherin at adherens junctions, mediating endothelial barrier function, and controlling vascular sprouting (5). In the immune system, CMTM4 acts as a backup for CMTM6 to regulate plasma membrane expression of PD-L1, an immune inhibitory ligand critical for immune tolerance to self and anti-tumor immunity (6-8). CMTM4 may also protect PD-L1 from being polyubiquitinated and targeted for degradation (8). Due to the roles of CMTM4 in the immune system and as a tumor suppressor, it is being investigated as a therapeutic target for the treatment of cancer. Alternate Names chemokine-like factor super family 4; Chemokine-like factor superfamily member 4; CKLF like MARVEL transmembrane domain containing 4; CKLF-like MARVEL transmembrane domain containing 4; CKLF-like MARVEL transmembrane domain-containing protein 4; CKLF4; CKLFSF4; CMTM4 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 22 SOURCE: Rabbit