CST, 17635SF, Notch2 (D76A6) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Notch2. Validated for WB,IHC,IF,F. Highly specific and rigorously validated in-house, Notch2 (D76A6) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #17635) is ready to ship. Product Usage Information This product is the carrier free version of product #5732. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 5732 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Notch2 (D76A6) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total Notch2 protein. Species cross-reactivity for IHC-P is human only. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala2378 of human Notch2 protein. Background Notch proteins (Notch1-4) are a family of transmembrane receptors that play important roles in development and the determination of cell fate (1). Mature Notch receptors are processed and assembled as heterodimeric proteins, with each dimer composed of a large extracellular ligand-binding domain, a single-pass transmembrane domain, and a smaller cytoplasmic subunit (Notch intracellular domain, NICD) (2). Binding of Notch receptors to ligands of the Delta-Serrate-Lag2 (DSL) family triggers heterodimer dissociation, exposing the receptors to proteolytic cleavages; these result in release of the NICD, which translocates to the nucleus and activates transcription of downstream target genes (3,4). Notch2 is a member of the Notch family and mutation in Notch2 is associated with Alagille syndrome (5). Alternate Names AGS2; HJCYS; hN2; N2ECD; N2ICD; Neurogenic locus notch homolog protein 2; NOTC2; Notch 2; Notch 2 extracellular truncation; Notch 2 intracellular domain; Notch homolog 2; Notch homolog 2 (Drosophila); notch receptor 2; NOTCH2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 110, 300 Source/Isotype: Rabbit