CST, 17636S, HMCES (F2J3S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying HMCES. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, HMCES (F2J3S) Rabbit Monoclonal Antibody (CST #17636) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity HMCES (F2J3S) Rabbit Monoclonal Antibody recognizes endogenous levels of total HMCES protein. This antibody detects approximately 65 kDa, 80 kDa, and 120 kDa proteins of unknown identity in some cell lines. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human HMCES protein. Background HMCES is an abasic site processing protein belonging to the SOS-response associated peptidase (SRAP) family (1,2). In DNA, loss of a base or nucleotide generates an abasic or apurinic/apyrimidinic (AP) site, one of the most common DNA lesions (1,2). HMCES plays a critical role in recognizing these abasic (AP) sites (1,2). HMCES directly binds proliferating cell nuclear antigen (PCNA) and single-stranded DNA (ssDNA) at replication forks, forming covalent cross-links to promote error-free genome repair (1-3). The HMCES DNA-protein cross-link (DPC) prevents translesion DNA synthesis and endonuclease activity, thereby stopping the generation of mutations and double-stranded DNA (dsDNA) breaks (2). Following this action, the HMCES-DPC is degraded by the proteasome or self-reversed (2,3). In dsDNA, AP sites are repaired via the base excision repair (BER) pathway (1-3). During somatic hypermutation (SHM) in B cells, HMCES suppresses deletions within immunoglobulin (Ig) genes but allows other types of point mutations to occur, resulting in antigen-specific high-affinity antibodies (4). HMCES deficiency impairs class switch recombination (CSR) in B cells, leading to weakened antibody production (5). In APOBEC3A-expressing tumors, disruption of HMCES may increase the tumor's sensitivity to therapies such as ionizing radiation (IR), oxidative stress, and ATR inhibition (6). Alternate Names 5-hydroxymethylcytosine (hmC) binding, ES cell-specific; 5-hydroxymethylcytosine binding, ES cell specific; Abasic site processing protein HMCES; C3orf37; DC12; Embryonic stem cell-specific 5-hydroxymethylcytosine-binding protein; ES cell-specific 5hmC-binding protein; HMCES; MGC111075; Peptidase HMCES; putative endonuclease HMCES; putative peptidase SRAPD1; SOS response associated peptidase domain containing 1; SRAP domain-containing protein 1; SRAPD1; UPF0361 protein C3orf37 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 40 Source/Isotype: Rabbit IgG