CST, 17688SF, CXCL13 (F6A4R) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying CXCL13. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, CXCL13 (F6A4R) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #17688) is ready to ship. Product Usage Information This product is the carrier free version of product #85679. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 85679 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity CXCL13 (F6A4R) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total CXCL13 protein. This antibody detects 58 and 75 kDa bands of unknown origin in some cell lines. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CXCL13 protein. Background C-X-C motif chemokine ligand 13 (CXCL13), also known as B cell-attracting chemokine 1 (BCA-1), is a chemokine expressed primarily by follicular dendritic cells of secondary lymphoid organs such as lymph nodes (LNs), Peyer's patches, and spleen (1,2). CXCL13 is the ligand for CXCR5, and upon binding, the CXCL13/CXCR5 axis is required for B cell activation, migration, and homeostasis (1-4). Dysregulated CXCL13 expression has been associated with autoimmunity, cancer, and infectious diseases, making it a potential therapeutic target (5). Alternate Names Angie; ANGIE2; B cell-attracting chemokine 1; B lymphocyte chemoattractant; B-cell chemoattractant; B-cell-attracting chemokine 1; B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1); B-lymphocyte chemoattractant; BCA-1; BCA1; BLC; BLR1L; C-X-C motif chemokine 13; C-X-C motif chemokine ligand 13; chemokine (C-X-C motif) ligand 13; chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant); CXC chemokine BLC; CXCL13; CXL13; SCYB13; small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cell chemoattractant); Small-inducible cytokine B13 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 13 Source/Isotype: Rabbit IgG