CST, 17700S, Complexin-1 (D5Q5H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying complexin-1. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Complexin-1 (D5Q5H) Rabbit Monoclonal Antibody (CST #17700) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Complexin-1 (D5Q5H) Rabbit Monoclonal Antibody recognizes endogenous levels of total Compexin-1 protein. This antibody does not cross-react with Complexin-2. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human Complexin-1 protein. Background Complexins are small soluble proteins composed of a central α-helical-structured domain surrounded by amino- and carboxy-terminal unstructured domains (1). These cytosolic proteins bind to t-SNAREs with low affinity and to assembled SNARE complexes with high affinity (1,2). Two isoforms, complexin-1 and complexin-2, are expressed in neuronal cells (3) where they regulate evoked and spontaneous exocytosis (4,5). Altered complexin expression resulting from RNAi-mediated knockdown (6) or gene invalidation (7) leads to alteration in spontaneous fusion events and neurotransmitter release, which reflects functions at both inhibitory and stimulatory synapses. Alternate Names complexin 1; Complexin I; Complexin-1; CPLX1; CPX I; CPX-I; CPX1; EIEE63; synaphin 2; Synaphin-2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 14 Source/Isotype: Rabbit IgG