CST, 17705T, GATAD2A (E7B5B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying GATAD2A. Validated for Western Blotting,Immunoprecipitation,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, GATAD2A (E7B5B) Rabbit Monoclonal Antibody (CST #17705) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunoprecipitation: 1:100 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP Specificity / Sensitivity GATAD2A (E7B5B) Rabbit Monoclonal Antibody recognizes endogenous levels of total GATAD2A protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro627 of human GATAD2A protein. Background GATAD2A and GATAD2B, also known as p66 alpha and p66 beta, are related members of the nucleosome remodeling and deacetylase (NuRD) complex (1). GATAD2A interacts with MBD2 to recruit the deacetylase core of the NuRD complex to repress target genes, a process that is enhanced by the sumoylation of GATAD2A at Lys30 and Lys487 (2-5). In addition to being sumoylated, GATAD2A has also been shown to be phosphorylated by AMPK in the memory formation process (6). Depletion of GATAD2A in order to reduce NuRD-dependent repression of key pluripotency genes is being explored in the context of induced pluripotent stem cell (iPSC) generation (7,8). Alternate Names FLJ20085; FLJ21017; GATA zinc finger domain containing 2A; GATA zinc finger domain-containing protein 2A; GATAD2A; Hp66alpha; p66 alpha; P66A; p66alpha; Transcriptional repressor p66-alpha Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 70, 73 Source/Isotype: Rabbit IgG