CST, 17821S, PARIS/ZNF746 (F7U7T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PARIS. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, PARIS/ZNF746 (F7U7T) Rabbit Monoclonal Antibody (CST #17821) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity PARIS/ZNF746 (F7U7T) Rabbit Monoclonal Antibody recognizes endogenous levels of total PARIS/ZNF746 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly435 of human PARIS/ZNF746 protein. Background Parkin-interacting substrate (PARIS), also known as Zinc finger protein 746 (ZNF746), is a tightly regulated transcriptional repressor. Structurally, PARIS/ZNF746 contains an N-terminal Krüppel-associated box (KRAB) domain and four zinc finger domains. The zinc finger domains promote DNA binding, while the KRAB domain mediates its repressor activity. PARIS/ZNF746 binds insulin response sequences (IRS) within the promoter region of peroxisome-proliferator activated receptor gamma coactivator-1 alpha (PGC-1 alpha), downregulating its expression (1,2). PGC-1 alpha is a master regulator of mitochondrial biogenesis, which is important for combating the buildup of reactive oxygen species (ROS) and maintaining cellular homeostasis. Aggregation of toxic protein species, including alpha-synuclein in Parkinson's disease (PD), leads to ROS buildup. PGC-1 alpha overexpression has been shown to be protective in PD models. PARIS/ZNF746 regulation of PGC-1 alpha expression is mediated by PTEN induced putative kinase 1 (PINK1) and Parkin. PINK1 phosphorylates PARIS/ZNF746, leading to ubiquitination by Parkin and degradation by the proteasome. Dysfunction or deletion of PINK1 or Parkin leads to PARIS accumulation, PGC-1 alpha repression, and the degeneration of dopaminergic neurons in PD models (3-5). Alternate Names FLJ31413; PARIS; Parkin-interacting substrate; parkin-interacting sustrate; Zinc finger protein 746; ZN746; ZNF746 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 95, 85 Source/Isotype: Rabbit IgG