{"product_id":"cst-17821s","title":"CST,  17821S, PARIS\/ZNF746 (F7U7T) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying PARIS. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, PARIS\/ZNF746 (F7U7T) Rabbit Monoclonal Antibody (CST #17821) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:50 - 1:250\nImmunoprecipitation: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPARIS\/ZNF746 (F7U7T) Rabbit Monoclonal Antibody recognizes endogenous levels of total PARIS\/ZNF746 protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly435 of human PARIS\/ZNF746 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nParkin-interacting substrate (PARIS), also known as Zinc finger protein 746 (ZNF746), is a tightly regulated transcriptional repressor. Structurally, PARIS\/ZNF746 contains an N-terminal Krüppel-associated box (KRAB) domain and four zinc finger domains. The zinc finger domains promote DNA binding, while the KRAB domain mediates its repressor activity. PARIS\/ZNF746 binds insulin response sequences (IRS) within the promoter region of peroxisome-proliferator activated receptor gamma coactivator-1 alpha (PGC-1 alpha), downregulating its expression (1,2). PGC-1 alpha is a master regulator of mitochondrial biogenesis, which is important for combating the buildup of reactive oxygen species (ROS) and maintaining cellular homeostasis. Aggregation of toxic protein species, including alpha-synuclein in Parkinson's disease (PD), leads to ROS buildup. PGC-1 alpha overexpression has been shown to be protective in PD models. PARIS\/ZNF746 regulation of PGC-1 alpha expression is mediated by PTEN induced putative kinase 1 (PINK1) and Parkin. PINK1 phosphorylates PARIS\/ZNF746, leading to ubiquitination by Parkin and degradation by the proteasome. Dysfunction or deletion of PINK1 or Parkin leads to PARIS accumulation, PGC-1 alpha repression, and the degeneration of dopaminergic neurons in PD models (3-5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nFLJ31413; PARIS; Parkin-interacting substrate; parkin-interacting sustrate; Zinc finger protein 746; ZN746; ZNF746\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 95, 85\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797635387561,"sku":"17821S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-17821s","provider":"Iright","version":"1.0","type":"link"}