CST, 17877T, INTS6 (F1S8S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying INTS6. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, INTS6 (F1S8S) Rabbit Monoclonal Antibody (CST #17877) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity INTS6 (F1S8S) Rabbit Monoclonal Antibody recognizes endogenous levels of total INTS6 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala441 of human INTS6 protein. Background The integrator complex is a multiprotein complex consisting of multiple subunits that regulates the termination of RNA polymerase II (RNAPII) transcription (1,2). The integrator was first discovered as a complex that processes the 3′ end of small nuclear RNAs, but subsequent studies have also established the importance of this complex in processing enhancer, viral, and telomeric RNAs (3). (integrator complex subunit 6), also known as (deleted in cancer 1), was first identified in non-small cell lung cancer cells and within the 13q14 chromosomal region (4). Given the susceptibility of this region to allelic deletions in many solid tumors, has been extensively examined as a tumor suppressor gene in humans (4-6). This protein was also identified as one of the integrator complex subunits (7). Its primary role is to facilitate protein phosphatase 2A (PP2A) assembly into the integrator-PP2A complex, ultimately contributing to target gene repression (8). Downregulation of INTS6 expression is associated with a poorer prognosis in hepatocellular carcinoma patients (9), while INTS6 levels were found to be significantly increased in colorectal carcinoma tissues and associated with poor prognosis (10). Alternate Names AL354820.3; DBI-1; DBI1; DDX26; DDX26A; DEAD box protein; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 26; DICE1; DKFZp434B105; HDB; Int6; Integrator complex subunit 6; INTS6; Notchl2; Protein DDX26; Protein deleted in cancer 1; RNA helicase HDB Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 120 Source/Isotype: Rabbit IgG