CST, 18012S, RBM10 Antibody

Polyclonal Antibody for studying RBM10. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, RBM10 Antibody (CST #18012) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity RBM10 Antibody recognizes endogenous levels of total RBM10 protein. Species Reactivity: Human, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly53 of human RBM10 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background RBM10 is an RNA binding protein and a paralog to tumor suppressor RBM5 (1,2). Alternative splicing creates two highly expressed variants that differ by one exon. RBM10 controls alternative splicing and exon skipping of mRNAs, including Fas and Bcl-x (3,4). RBM10 has been shown to affect apoptosis via up regulation of TNF-α mRNA (5). In lung cancer, RBM10 mutations disrupt the splicing of NUMB, a regulator of Notch signaling (6,7). Fusion of the gene with TFE3 has been found in renal cell carcinoma (8-9). Mutations of RBM10 causes TARP syndrome, an x-linked, lethal disorder characterized by various developmental defects (10-12). Alternate Names DXS8237E; epididymis secretory sperm binding protein; G patch domain-containing protein 9; GPATC9; GPATCH9; KIAA0122; MGC1132; MGC997; RBM10; RNA binding motif protein 10; RNA-binding motif protein 10; RNA-binding protein 10; RNA-binding protein S1-1; S1-1; TARPS; ZRANB5 Specification REACTIVITY: H R Mk SENSITIVITY: Endogenous MW (kDa): 110, 135 SOURCE: Rabbit