{"product_id":"cst-18215t","title":"CST,  18215T, BRCC36 (D5E5H) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying BRCC36. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, BRCC36 (D5E5H) Rabbit Monoclonal Antibody (CST #18215) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nBRCC36 (D5E5H) Rabbit Monoclonal Antibody recognizes endogenous levels of total BRCC36 protein. This antibody detects BRCC36 isoform1 and BRCC36 isoform2.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His251 of human BRCC36 isoform 2 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nProtein ubiquitination and deubiquitination are reversible processes catalyzed by ubiquitinating enzymes and deubiquitinating enzymes, respectively (1,2). Deubiquitinating enzymes (DUBs) are categorized into five subfamilies based on catalytic domain structure: USP, OTU, MJD, UCH, and JAMM\/MPN. BRCC36 is a zinc-dependent DUB belonging to the JAMM\/MPN subfamily and participates in DNA damage responses and interferon signaling by specifically catalyzing hydrolysis of K63-linked polyubiquitin chains (3,4). In the nucleus, BRCC36 is part of the BRCA1-A complex that contains RAP80, BRCA1, ABRAXAS, and MERIT40 (5,6). This complex plays a critical role in mediating the cellular repair of DNA double strand breaks induced by ionizing radiation (7-10). Research studies have shown that BRCC36 is overexpressed in a high percentage of breast tumors, which may contribute to resistance of breast cancer cells to ionizing radiation-induced apoptosis (4). BRCC36 also functions in the cytoplasm as part of a distinct complex known as the BRCC36-containing isopeptide complex (BRISC) (5,6). Indeed, research studies have shown that BRCC36 deubquitinates and stabilizes IFNAR1 to modulate the cellular response to interferons (3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nBRCA1-A complex subunit BRCC36; BRCA1\/BRCA2-containing complex subunit 3; BRCA1\/BRCA2-containing complex subunit 36; BRCA1\/BRCA2-containing complex, subunit 3; BRCC3; BRCC36; BRISC complex subunit BRCC36; C6.1A; CXorf53; Lys-63-specific deubiquitinase BRCC36\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 33, 40\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797636272297,"sku":"18215T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-18215t","provider":"Iright","version":"1.0","type":"link"}