CST, 18269S, Alix (E4T7U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Alix. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Alix (E4T7U) Rabbit Monoclonal Antibody (CST #18269) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Alix (E4T7U) Rabbit Monoclonal Antibody recognizes endogenous levels of total Alix protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg475 of human Alix protein. Background Alix, a phylogenetically conserved cytosolic scaffold protein, contains an N-terminal Bro1 domain, a coiled-coil region and a C-terminal proline-rich domain (1,2). Originally identified as an ALG-2 (apoptosis-linked gene 2)-interacting protein involved in programmed cell death (3,4), Alix also regulates many other cellular processes, such as endocytic membrane trafficking and cell adhesion through interactions with ESCRT (endosomal sorting complex required for transport) proteins, endophilins, and CIN85 (Cbl-interacting protein of 85 kDa) (5,6). Alix has also been shown to be involved in the biogenesis of exosomes, small secreted vesicles that contribute to cell signaling (7, 8). In addition, Alix interacts with Atg12-Atg3 to promote autophagy and endosomal fusion (9) Alternate Names AIP1; ALG-2 interacting protein 1; ALG-2 interacting protein X; ALG-2-interacting protein 1; ALG-2-interacting protein X; ALIX; apoptosis-linked gene 2-interacting protein X; dopamine receptor interacting protein 4; DRIP4; Hp95; KIAA1375; MGC17003; PDC6I; PDCD6-interacting protein; PDCD6IP; programmed cell death 6 interacting protein; Programmed cell death 6-interacting protein Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 90-100 Source/Isotype: Rabbit IgG