{"product_id":"cst-18381t","title":"CST,  18381T, HSL (D6W5S) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying HSL. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, HSL (D6W5S) Rabbit Monoclonal Antibody (CST #18381) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\nImmunofluorescence (Immunocytochemistry): 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nHSL (D6W5S) Rabbit Monoclonal Antibody recognizes endogenous levels of total HSL protein.\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His946 of human HSL protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nHSL (hormone-sensitive lipase) catalyzes the hydrolysis of triacylglycerol, the rate-limiting step in lipolysis. Lipolytic stimuli activate adenylyl cyclase and thus increase intracellular cAMP levels, which in turn activate protein kinase A (PKA). PKA phosphorylates HSL at Ser563, Ser659, and Ser660, which stimulates HSL activity (1,2). In contrast, AMPK phosphorylates HSL at Ser565, which reduces HSL phosphorylation at Ser563 by PKA and inhibits HSL activity (2,3). Recent work indicates that phosphorylation at Ser600 by p44\/42 MAPKs also enhances the enzymatic activity of HSL (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAOMS4; FPLD6; Hormone-sensitive lipase; hormone-sensitive lipase testicular isoform; HSL; LHS; lipase E, hormone sensitive type; lipase, hormone-sensitive; LIPE; LIPS; Monoacylglycerol lipase LIPE; REH; Retinyl ester hydrolase\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nMW (kDa): 81, 83\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797636731049,"sku":"18381T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-18381t","provider":"Iright","version":"1.0","type":"link"}