CST, 18420T, ECHS1 (F4A1Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ECHS1. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, ECHS1 (F4A1Z) Rabbit Monoclonal Antibody (CST #18420) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity ECHS1 (F4A1Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total ECHS1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ECHS1 protein. Background Mitochondrial enoyl-CoA hydratase short chain 1 (ECHS1), also known as short-chain enoyl-CoA hydratase (SCEH), is active in many metabolic pathways, including short-chain fatty acid β-oxidation and branched-chain amino acid catabolic pathway (1-3). Studies show that lysine catabolism in glioblastoma stem cells (GSCs) is altered via upregulation of lysine transporter SLC7A2 and crotonyl-CoA-producing enzyme GCDH and downregulation of ECHS1. These changes result in histone H4 lysine crotonylation. Lysine restriction decreases histone lysine crotonylation and reduces tumor growth (3). Alternate Names ECHM; ECHS1; ECHS1D; enoyl Coenzyme A hydratase, short chain, 1, mitochondrial; Enoyl-CoA hydratase 1; Enoyl-CoA hydratase, mitochondrial; enoyl-CoA hydratase, short chain 1; enoyl-CoA hydratase, short chain, 1, mitochondrial; epididymis secretory sperm binding protein; SCEH; short chain enoyl-CoA hydratase; Short-chain enoyl-CoA hydratase Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 25 Source/Isotype: Rabbit IgG