{"product_id":"cst-18420t","title":"CST,  18420T, ECHS1 (F4A1Z) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying ECHS1. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, ECHS1 (F4A1Z) Rabbit Monoclonal Antibody (CST #18420) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:50 - 1:250\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nECHS1 (F4A1Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total ECHS1 protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ECHS1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nMitochondrial enoyl-CoA hydratase short chain 1 (ECHS1), also known as short-chain enoyl-CoA hydratase (SCEH), is active in many metabolic pathways, including short-chain fatty acid β-oxidation and branched-chain amino acid catabolic pathway (1-3). Studies show that lysine catabolism in glioblastoma stem cells (GSCs) is altered via upregulation of lysine transporter SLC7A2 and crotonyl-CoA-producing enzyme GCDH and downregulation of ECHS1. These changes result in histone H4 lysine crotonylation. Lysine restriction decreases histone lysine crotonylation and reduces tumor growth (3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nECHM; ECHS1; ECHS1D; enoyl Coenzyme A hydratase, short chain, 1, mitochondrial; Enoyl-CoA hydratase 1; Enoyl-CoA hydratase, mitochondrial; enoyl-CoA hydratase, short chain 1; enoyl-CoA hydratase, short chain, 1, mitochondrial; epididymis secretory sperm binding protein; SCEH; short chain enoyl-CoA hydratase; Short-chain enoyl-CoA hydratase\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 25\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797636894889,"sku":"18420T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-18420t","provider":"Iright","version":"1.0","type":"link"}