CST, 18553S, Caprin1 (E9F9I) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying caprin1. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, Caprin1 (E9F9I) Rabbit Monoclonal Antibody (CST #18553) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Caprin1 (E9F9I) Rabbit Monoclonal Antibody recognizes endogenous levels of total caprin1 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu201 of human caprin1 protein. Background Cytoplasmic activation- and proliferation-associated protein 1 (caprin1) is a ubiquitously expressed RNA binding protein (RBP) that plays an important role in cell cycle regulation. Caprin1 is primarily localized to the cytoplasm and interacts with mRNA through its C-terminal RGG motifs (1). Caprin1 interacts with a variety of mRNA involved in cell cycle regulation, including c-Myc and cyclin D2 (1,2). Additionally, knockout of caprin1 reduces cell proliferation by delaying the transition from the G1 to the S phase of the cell cycle (3). Misregulation of the cell cycle suggests a potential tumorigenic role for caprin1, and its overexpression has been found to promote tumor growth and metastasis in osteosarcoma through interaction with Cyr61 (4). Caprin1 is also known to phase separate and localize to specific RNA granules called stress granules (SGs). SGs are cytoplasmic mRNA-protein condensates that are formed in response to cellular stressors, such as oxidative stress, ultraviolet radiation, and viral infection (5). Caprin1 is a key mediator of SG formation along with the proteins G3BP1 and USP10 (6,7). SG formation is a reversible process under normal physiological conditions, but chronic stress can disrupt SG dissolution and lead to the aggregation of proteins linked to various neurodegenerative diseases (8). Indeed, a missense P512L mutation in the gene has been linked to early-onset ataxia, potentially through a gain-of-function mechanism that promotes protein aggregation (9). Alternate Names activation/proliferation-associated protein 1; CAPR1; caprin 1; Caprin-1; CAPRIN1; cell cycle associated protein 1; Cell cycle-associated protein 1; Cytoplasmic activation- and proliferation-associated protein 1; cytoplasmic activation/proliferation-associated protein-1; GPI-anchored membrane protein 1; GPI-anchored protein p137; GPI-p137; GPIAP1; GPIP137; GRIP137; M11S1; Membrane component chromosome 11 surface marker 1; membrane component, chromosome 11, surface marker 1; p137GPI; RNA granule protein 105; RNG105 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 116 Source/Isotype: Rabbit IgG