CST, 18582T, FKBP8 (E6F3T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FKBP8. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, FKBP8 (E6F3T) Rabbit Monoclonal Antibody (CST #18582) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity FKBP8 (E6F3T) Rabbit Monoclonal Antibody recognizes endogenous levels of total FKBP8 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu72 of human FKBP8 protein. Background FKBP8, also known as FKBP38, is a member of the FK506-binding protein (FKBP) family that interacts with the immunosuppressive drug FK506 through a conserved peptidyl-prolyl isomerase (PPIase) domain (1,2). In contrast to other FKBP family members, the PPIase activity of FKBP8 is dependent on Ca -signaling through a Ca -calmodulin binding domain (3). Loss of FKBP8 leads to defects in neural tube and eye development (4,5). FKBP family members generally function as molecular chaperones interacting with distinct proteins (2). FKBP8 is predominantly localized to the endoplasmic reticulum and the mitochondrial outer membrane and has been found to interact with proteins controlling apoptosis, including Bcl-2 and Bcl-xL (6). In addition, FKBP8 was found to promote autophagy through association with Beclin-1, a key component for activation of the VPS34 lipid kinase required for initiation of autophagy (7). FKBP8 also promotes autophagy by inhibiting mTOR, a kinase that plays a key role in cell growth (8). FKBP8 can also play a role in the clearance of damaged mitochondria through mitophagy. It contains an amino-terminal LC3-interacting region (LIR) motif that binds strongly to LC3A and recruits lipidated LC3A to damaged mitochondria to induce Parkin-independent mitophagy and promote mitochondrial fragmentation (9,10). Alternate Names 38 kDa FK506-binding protein; 38 kDa FKBP; FK506 binding protein 8, 38kDa; FK506-binding protein 8; FK506-binding protein 8 (38kD); FKBP prolyl isomerase 8; FKBP-38; FKBP-8; FKBP38; FKBP8; FKBPR38; hFKBP38; Peptidyl-prolyl cis-trans isomerase FKBP8; PPIase FKBP8; Rotamase Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 55 Source/Isotype: Rabbit IgG