CST, 18794T, CD272/BTLA (F2U1F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying BTLA. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, CD272/BTLA (F2U1F) Rabbit Monoclonal Antibody (CST #18794) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CD272/BTLA (F2U1F) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD272/BTLA protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human CD272/BTLA protein. Background B and T lymphocyte attenuator (BTLA), also known as CD272, is a type 1 transmembrane glycoprotein and is a member of the CD28 immunoglobulin (Ig) superfamily. BTLA has an extracellular IgV domain, and the cytoplasmic domain is made up of a growth factor receptor-bound protein 2 (GRB2) association motif, an immunoreceptor tyrosine-based inhibitory motif (ITIM), and an immunoreceptor tyrosine-based switch motif (ITSM) (1-3). BTLA is also expressed in a soluble form (sBTLA), produced as a result of alternative splicing (4). BTLA is mainly expressed on B and T cells and has been observed on murine type 1 conventional dendritic cells (cDC1) (5). BTLA interacts with its ligand Herpesvirus entry mediator (HVEM), a member of the TNF receptor superfamily, which induces phosphorylation of ITIMs and Src homology 2 (SH2) domain-containing phosphatase 1 (SHP-1)/SHP-2 association leading to repression of T cell proliferation and cytokine production (6,7). GRB2 binding to the GRB2 association motif leads to the recruitment of PI3K protein subunit p85 and T cell activation (1). BTLA expression is one of the markers used to help identify exhausted and dysfunctional lymphocytes and often correlates with cancer progression (8). Elevated sBTLA plasma levels are associated with sepsis, autoimmune disease, and cancer (9,10). The BTLA-HVEM axis is an important immune checkpoint in CAR-T cell immunotherapy, and strategies to overcome this hurdle are under investigation (11). Alternate Names B and T lymphocyte associated; B and T lymphocyte attenuator; B- and T-lymphocyte attenuator; B- and T-lymphocyte-associated protein; BTLA; BTLA1; CD272; FLJ16065; MGC129743 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 20-25, 42-60 Source/Isotype: Rabbit IgG