{"product_id":"cst-18946s","title":"CST,  18946S, VISTA (D1L2G™) Rabbit Monoclonal Antibody (PE Conjugate)","description":"Monoclonal Antibody for studying VISTA. Validated for Flow Cytometry (Fixed\/Permeabilized). Highly specific and rigorously validated in-house, VISTA (D1L2G\u003csup\u003e™\u003c\/sup\u003e) Rabbit Monoclonal Antibody (PE Conjugate) (CST #18946) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFlow Cytometry (Fixed\/Permeabilized): 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg\/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nVISTA (D1L2G™) Rabbit Monoclonal Antibody (PE Conjugate) recognizes endogenous levels of total VISTA protein.\nSpecies Reactivity: Human, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human VISTA protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nVISTA (V-Domain Ig Suppressor of T Cell Activation) is a negative checkpoint control protein that regulates T cell activation and immune responses. VISTA, which contains a single Ig-like V-type domain, a transmembrane domain, and an intracellular domain, has sequence similarity to both the B7 and CD28 family members. Although primarily expressed by myeloid cells, VISTA is also expressed by CD4+, CD8+, and FoxP3+ T-cells. Thus, VISTA is described as both a ligand and a receptor (1-3). Blocking VISTA induces T-cell activation and proliferation, and potentiates disease severity in the EAE model (1). Furthermore, genetic deletion of VISTA in mice leads to spontaneous T-cell activation and chronic inflammation (4,5). In mouse models of cancer, neutralization of VISTA enhances T-cell proliferation and effector function and increases tumor infiltration, suggesting VISTA blockade could be an effective strategy for tumor immunotherapy (6,7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nB7-H5; B7H5; C10orf54; DD1alpha; Death Domain1alpha; Dies1; GI24; PD-1H; PDCD1 homolog; Platelet receptor Gi24; PP2135; Sisp-1; SISP1; stress induced secreted protein 1; Stress-induced secreted protein-1; V-domain Ig suppressor of T cell activation; V-set domain-containing immunoregulatory receptor; V-set immunoregulatory receptor; V-type immunoglobulin domain-containing suppressor of T-cell activation; VISTA; VSIR\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H Mk\nSENSITIVITY: Endogenous\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800355393705,"sku":"18946S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-18946s","provider":"Iright","version":"1.0","type":"link"}