{"product_id":"cst-19056s","title":"CST,  19056S, Tuberin\/TSC2 (D93F12) Rabbit Monoclonal Antibody (Biotinylated)","description":"Monoclonal Antibody for studying Tuberin\/TSC2. Validated for Western Blotting. Highly specific and rigorously validated in-house, Tuberin\/TSC2 (D93F12) Rabbit Monoclonal Antibody (Biotinylated) (CST #19056) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 140 mM NaCl, 3 mM KCI, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg\/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nTuberin\/TSC2 (D93F12) Rabbit Monoclonal Antibody (Biotinylated) detects endogenous levels of total tuberin protein.\nSpecies Reactivity: Human, Mouse, Rat, Hamster, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues of human tuberin protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nTuberin is a product of the TSC2 tumor suppressor gene and an important regulator of cell proliferation and tumor development (1). Mutations in either or the related (hamartin) gene cause tuberous sclerosis complex (TSC), an autosomal dominant disorder characterized by development of multiple, widespread non-malignant tumors (2). Tuberin is directly phosphorylated at Thr1462 by Akt\/PKB (3). Phosphorylation at Thr1462 and Tyr1571 regulates tuberin-hamartin complexes and tuberin activity (3-5). In addition, tuberin inhibits the mammalian target of rapamycin (mTOR), which promotes inhibition of p70 S6 kinase, activation of eukaryotic initiation factor 4E binding protein 1 (4E-BP1, an inhibitor of translation initiation), and eventual inhibition of translation (3,6,7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nFLJ43106; LAM; PPP1R160; protein phosphatase 1, regulatory subunit 160; TSC complex subunit 2; TSC2; TSC4; Tuberin; tuberous sclerosis 2; Tuberous sclerosis 2 protein\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Hm Mk\nSENSITIVITY: Endogenous\nMW (kDa): 200\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800355721385,"sku":"19056S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-19056s","provider":"Iright","version":"1.0","type":"link"}