{"product_id":"cst-19156t","title":"CST,  19156T, ASC\/TMS1 (D2W8U) Horse Chimeric Monoclonal Antibody","description":"Monoclonal Antibody for studying ASC mouse. Validated for Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, ASC\/TMS1 (D2W8U) Horse Chimeric Monoclonal Antibody (CST #19156) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nImmunofluorescence (Frozen): 1:50 - 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Immunofluorescence (Frozen)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nASC\/TMS1 (D2W8U) Horse Chimeric Monoclonal Antibody recognizes endogenous levels of total ASC\/TMS1 protein.\nSpecies Reactivity: Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nThis recombinant chimeric antibody is engineered from ASC\/TMS1 (D2W8U) Rabbit mAb #67824 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody but contains a horse-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies. The parent antibody, ASC\/TMS1 (D2W8U) Rabbit mAb #67824, is produced by immunizing animals with recombinant mouse ASC\/TMS1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nTMS1 (target of methylation-induced silencing)\/ASC (apoptosis-associated speck-like protein containing a CARD), also referred to as PYCARD and CARD5, is a 22 kDa pro-apoptotic protein containing an N-terminal pyrin domain (PYD) and a C-terminal caspase recruitment domain (CARD) (1-2). The gene was originally found to be aberrantly methylated and silenced in breast cancer cells (2), and has since been found to be silenced in a number of other cancers, including ovarian cancer (3), glioblastoma (4), melanoma (5), gastric cancer (6), lung cancer (7), and prostate cancer (8). Expression of ASC\/TMS1 can be induced by pro-apoptotic\/inflammatory stimuli (9). During apoptosis ASC\/TMS1 is redistributed from the cytosol to the mitochondria and associates with mitochondrial Bax to trigger cytochrome c release and subsequent apoptosis (10). ASC\/TMS1 has also been found to be a critical component of inflammatory signaling where it associates with and activates caspase-1 in response to pro-inflammatory signals (11).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n9130417A21Rik; Apoptosis-associated speck-like protein containing a CARD; Asc; CARD5; mASC; OTTMUSP00000030782; Pycard; PYD and CARD domain containing; PYD and CARD domain-containing protein; TMS-; TMS-1; TNS1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M\nSENSITIVITY: Endogenous\nSource\/Isotype: Horse chimeric IgG4","brand":"CST","offers":[{"title":"Default Title","offer_id":46800292085929,"sku":"19156T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-19156t","provider":"Iright","version":"1.0","type":"link"}