CST, 19168S, MARCH5 Antibody

Polyclonal Antibody for studying MARCH5. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, MARCH5 Antibody (CST #19168) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity MARCH5 Antibody recognizes endogenous levels of total MARCH5 protein. A band of unknown origin is detected at 55 kDa. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MARCH5 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Changes in mitochondrial dynamics regulated by environmental cues affect mitochondrial size and shape that regulate metabolism and cell death, and have significant consequences on health and disease (1,2). These processes are largely controlled by mitochondrial dynamin-related GTPases and associated proteins, including mitofusin-1 (MFN1), mitofusin-2 (MFN2), OPA1, MFF, Fis1, and DRP1. DRP1, MFF, and Fis1 regulate mitochondrial fission, while the mitofusins and OPA1 control fusion. MARCH5/MARCH-V/MITOL is a ubiquitin E3 ligase localized to the mitochondrial outer membrane that affects mitochondrial dynamics (3-5). MARCH5 has been associated with tumorigenesis, innate immunity, autophagy, apoptosis, and senescence (3-9). Mechanistically, MARCH5 associates with MFN2 and also promotes the ubiquitination of fission proteins DRP1, as well as hFis1 and MFN1 (3-6). Dysregulation of MARCH5 through overexpression, mutation, or knockdown can regulate the turnover of fission and fusion proteins and lead to changes in mitochondrial dynamics (3-6). Studies have shown that elevated levels of MARCH5 in ovarian and breast cancers potentially contribute to tumor growth and metastasis (10,11). Alternate Names E3 ubiquitin-protein ligase MARCH5; E3 ubiquitin-protein ligase MARCHF5; FLJ20445; MARCH-V; MARCH5; MARCHF5; MARH5; membrane associated ring finger 5; membrane associated ring-CH-type finger 5; membrane-associated ring finger (C3HC4) 5; Membrane-associated RING finger protein 5; Membrane-associated RING-CH protein V; mitochondrial ubiquitin ligase; MITOL; RING finger protein 153; RING-type E3 ubiquitin transferase MARCH5; RING-type E3 ubiquitin transferase MARCHF5; RNF153 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 25 SOURCE: Rabbit