CST, 19230S, LAMP5 (F9Q8X) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying LAMP5. Validated for WB,WB,IP,F. Highly specific and rigorously validated in-house, LAMP5 (F9Q8X) Rabbit Monoclonal Antibody (CST #19230) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity LAMP5 (F9Q8X) Rabbit Monoclonal Antibody recognizes endogenous levels of total LAMP5 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human LAMP5 protein. Background Lysosome-associated membrane glycoproteins (LAMPs) are a family of proteins present predominantly on the membrane of lysosomes. Five members have been identified so far (LAMP1, LAMP2, LAMP3, CD68/Macrosialin/LAMP4, and BAD-LAMP/LAMP5). LAMP proteins are involved in many different aspects of cell biology and can influence cellular processes, such as phagocytosis, autophagy, lipid transport, and aging. Their expression varies among different tissues (1). Differing from its family members, LAMP5, also called brain and dendritic cell-associated LAMP (BAD-LAMP), is almost exclusively expressed in the central nervous system (CNS). LAMP5 acts as a marker for a subset of GABAergic interneurons where it functions in synaptic transmission and synaptic plasticity, and has been shown to play a role in sensorimotor processing in the brain and spinal cord (2,3). Loss of LAMP5 positive interneurons can be seen in Alzheimer's disease, potentially implicating these cells in neurodegeneration (4). In addition to its role in the CNS, LAMP5 expression has been implicated in several cancer types, including gastric cancer, where it promotes metastatic potential of gastric cancer cells, and mixed lineage leukemia, where it acts as a suppressor of autophagy (5,6). Alternate Names BAD-LAMP; BADLAMP; Brain and dendritic cell-associated LAMP; Brain-associated LAMP-like protein; C20orf103; LAMP family protein C20orf103; LAMP-5; LAMP5; lysosomal associated membrane protein family member 5; lysosomal-associated membrane protein family, member 5; Lysosome-associated membrane glycoprotein 5; Lysosome-associated membrane protein 5; OTTHUMP00000030243; Q9UJQ1; UNC-43; UNC-46 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 31 Source/Isotype: Rabbit IgG