CST, 19472S, Cyclin K (E7F4N) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Cyclin K. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Cyclin K (E7F4N) Rabbit Monoclonal Antibody (CST #19472) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Cyclin K (E7F4N) Rabbit Monoclonal Antibody recognizes endogenous levels of total cyclin K protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala15 of human cyclin K protein. Background Cyclin K is a positive regulator of transcriptional cyclin-dependent kinases (CDKs) CDK12 and CDK13. The cyclin K/CDK12 complex regulates the formation of the pre-replicative complex during G1 phase of the cell cycle (1), as well as the expression of key proteins in the DNA Damage Response (DDR) (2). Regulation of DDR-related gene expression involves cyclin K interaction with the histone methyltransferase SETD1A (3). Due to its importance in maintaining genome stability through regulation of the DDR, the cyclin K/CDK12 complex is an emerging therapeutic target in human cancer (4,5). Targeted protein degradation via molecular glues and PROTACs have been employed to specifically degrade cyclin K/CDK12 as a means of targeting the DDR in human cancer (6-9). Alternate Names CCNK; CPR4; cyclin K; Cyclin-K; IDDHDF; MGC9113 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 79 Source/Isotype: Rabbit IgG