CST, 19582S, TSLPR/CRLF2 (F3K9G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CRLF2. Validated for WB,IP,F,F. Available in 2 sizes. Highly specific and rigorously validated in-house, TSLPR/CRLF2 (F3K9G) Rabbit Monoclonal Antibody (CST #19582) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Flow Cytometry (Fixed/Permeabilized): 1:200 - 1:800 Flow Cytometry (Live): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live) Specificity / Sensitivity TSLPR/CRLF2 (F3K9G) Rabbit Monoclonal Antibody recognizes endogenous levels of total TSLPR/CRLF2 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human TSLPR/CRLF2 protein. Background Thymic stromal lymphopoietin (TSLP) is a cytokine produced by epithelial cells at barrier surfaces and granulocytes (1). TSLP acts on dendritic cells, lymphocytes, and granulocytes by binding to a heterodimer composed of TSLPR (TSLP receptor; also known as cytokine receptor-like factor 2 or CRLF2) and IL-7Rα (2-5). Activation of this heterodimer further activates the Jak/Stat signaling pathway and stimulates cellular proliferation (6-8). TSLPR/CRLF2 overexpression is linked to leukemia, where it is identified in 5-15% of all cases of B-cell acute lymphoblastic leukemia (B-ALL) and is the most common hallmark of the Philadelphia chromosome (Ph)-like B-ALL subtype (8-11). This overexpression is associated with poor clinical outcome, and TSLPR/CRLF2 is increasingly being studied as a target for B-ALL immunotherapies (12,13). Alternate Names CRL2; CRLF2; CRLF2Y; cytokine receptor CRL2; cytokine receptor CRL2 precusor; cytokine receptor like factor 2; Cytokine receptor-like 2; Cytokine receptor-like factor 2; IL-XR; ILXR; P2RY8/CRLF2 fusion partner; Thymic stromal lymphopoietin protein receptor; thymic stromal lymphopoietin receptor; thymic stromal-derived lymphopoietin receptor; TSLP receptor; TSLPR Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 60 Source/Isotype: Rabbit IgG