CST, 19673SF, CD7 (E4G1Q) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying CD7. Validated for Western Blotting,IHC Leica Bond,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, CD7 (E4G1Q) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #19673) is ready to ship. Product Usage Information This product is the carrier free version of product #32814. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 32814 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity CD7 (E4G1Q) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total CD7 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu59 of human CD7 protein. Background CD7 is a type-I transmembrane glycoprotein belonging to the immunoglobulin superfamily. CD7 is one of the earliest surface markers to be expressed on the surface of developing T cells, and its expression is maintained throughout maturation of multiple T cell subsets and NK cells (1-3). Engagement of CD7 through binding its ligand, SECTM1, has been shown to promote tyrosine phosphorylation of its cytoplasmic domain, recruitment of PI3K, and delivery of costimulatory signals for T cell activation (4-6). While CD7 is expressed on normal T cells, it is also highly expressed in a variety of T cell malignancies, which has poised it as a potential target of immunotherapy (7-9). Alternate Names CD7; CD7 antigen; CD7 antigen (p41); CD7 molecule; GP40; LEU-9; p41 protein; T-cell antigen CD7; T-cell leukemia antigen; T-cell surface antigen Leu-9; Tp40; TP41 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 38-40 Source/Isotype: Rabbit IgG