CST, 19758S, USP39 Antibody

Polyclonal Antibody for studying USP39. Validated for Western Blotting. Highly specific and rigorously validated in-house, USP39 Antibody (CST #19758) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity USP39 Antibody recognizes endogenous levels of total USP39 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val88 of human USP39 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Ubiquitin specific protease 39 (USP39) is a 65 kDa protein that plays an important role in pre-mRNA splicing, as well as mitotic spindle formation. It displays significant homology with ubiquitin C-terminal hydrolase proteins (UCHs), containing both an N-terminal zinc finger domain as well as UCH-1 and UCH-2-like domains also observed in the UCH2 family of proteins (1). However, USP39 lacks a catalytic cysteine residue found in UCHs and has been shown experimentally to lack peptidase activity (2). USP39 associates with the U4/U6-U5 tri-small nuclear ribonucleoprotein (U4/U6-U5 tri-snRNP) complex and is necessary for the formation of the mature spliceosome. Silencing of USP39 has been shown to adversely affect chromosome segregation and cytokinesis in U2OS cells, likely due to improper splicing of Aurora B and other mRNAs necessary for mitotic spindle formation and checkpoint function (2). In addition, USP39 has been found to be overexpressed in many types of cancers, and in most cases is associated with tumor progression and poor prognosis. Overexpression has been observed in pancreatic (3), prostate (4), colorectal (5,6), lung (6,7), gastric (8), and triple negative breast cancers (9), as well as melanoma (10) and hepatocellular carcinoma (11,12). Alternate Names 65K; CGI-21; HSPC332; Inactive ubiquitin-specific peptidase 39; MGC75069; SAD1; SAD1 homolog; small nuclear ribonucleoprotein 65kDa (U4/U6.U5); SnRNP assembly defective 1 homolog; SNRNP65; SNUT2; U4/U6.U5 tri-snRNP-associated 65 kDa protein; U4/U6.U5 tri-snRNP-associated protein 2; Ubiquitin carboxyl-terminal hydrolase 39; ubiquitin specific peptidase 39; ubiquitin specific protease 39; USP39 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 65 SOURCE: Rabbit