{"product_id":"cst-19807s","title":"CST,  19807S, Non-phospho (Active) beta-Catenin (Ser45) (D2U8Y) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Catenin-beta (Thr41\/Ser45) non-phosphate. Validated for WB,IP,IHC,IF,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, Non-phospho (Active) beta-Catenin (Ser45) (D2U8Y) Rabbit Monoclonal Antibody (CST #19807) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nImmunohistochemistry (Paraffin): 1:500 - 1:2000\nImmunofluorescence (Frozen): 1:1600 - 1:6400\nImmunofluorescence (Immunocytochemistry): 1:1600 - 1:6400\nFlow Cytometry (Fixed\/Permeabilized): 1:800 - 1:3200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 24710 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nNon-phospho (Active) beta-Catenin (Ser45) (D2U8Y) Rabbit Monoclonal Antibody recognizes endogenous levels of β-catenin protein only when Ser45 is not phosphorylated.\nSpecies Reactivity: Human, Mouse, Rat, Monkey, Zebrafish, Dog\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser45 of human β-catenin protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nβ-catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8). Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP Rabbit mAb specifically recognizes β-catenin that is non-phosphorylated at Ser45. Non-phospho β-Catenin (Ser45) protein is useful as a readout of stabilized β-catenin protein, and therefore functionally active in cell-cell adhesion and mediating transcriptional activity via the canonical Wnt signaling pathway (9).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\narmadillo; Beta-catenin; catenin (cadherin-associated protein), beta 1, 88kDa; catenin beta 1; Catenin beta-1; CTNB1; CTNNB; CTNNB1; DKFZp686D02253; EVR7; FLJ25606; FLJ37923; MRD19; NEDSDV\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk Z Dg\nSENSITIVITY: Endogenous\nMW (kDa): 92\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797642694825,"sku":"19807S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-19807s","provider":"Iright","version":"1.0","type":"link"}