CST, 19944S, BAFF (D7I1U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying BAFF. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, BAFF (D7I1U) Rabbit Monoclonal Antibody (CST #19944) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity BAFF (D7I1U) Rabbit Monoclonal Antibody recognizes endogenous levels of total BAFF protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant human BAFF protein. Background BAFF, a member of the TNF superfamily of proteins, is a homotrimeric transmembrane protein, which is cleaved to produce a soluble cytokine (1). BAFF may also further oligomerize into 60-mer structures (1). BAFF is expressed by monocytes, neutrophils, macrophages, dendritic cells, activated T cells, and epithelial cells (1,2). BAFF plays a key role in B cell development, survival, and activation (1,3,4). BAFF binds to three distinct receptors, BAFF-R, TACI, and BCMA (1). These receptors are differentially expressed during B cell development and among B cell subsets (1,2,4). While BAFF-R and BCMA bind to the homotrimeric form of BAFF, TACI only binds to membrane bound or higher order BAFF structures (1). The BAFF/ BAFF-R interaction activates both canonical and non-canonical NF-κB pathways, PI3K/Akt, and mTOR (2,4). Activation of the noncanonical NF-κB pathway via BAFF-R is negatively regulated by TRAF3 (5). Research studies have shown that elevated levels of BAFF may exacerbate many autoimmune disorders, making it a potential therapeutic target (2). Alternate Names ApoL related ligand TALL-1; B lymphocyte stimulator; B-cell activating factor; B-cell-activating factor; B-lymphocyte stimulator; BAFF; BLYS; CD257; delta BAFF; Delta4 BAFF; Dendritic cell-derived TNF-like molecule; DTL; epididymis secretory sperm binding protein; TALL-1; TALL1; THANK; TN13B; TNF and ApoL-related leukocyte expressed ligand 1; TNF homolog that activates apoptosis; TNF superfamily member 13b; TNF- and APOL-related leukocyte expressed ligand 1; TNFSF13B; TNFSF20; TNLG7A; tumor necrosis factor (ligand) superfamily, member 13b; tumor necrosis factor (ligand) superfamily, member 20; tumor necrosis factor ligand 7A; Tumor necrosis factor ligand superfamily member 13B; Tumor necrosis factor ligand superfamily member 13b, membrane form; Tumor necrosis factor ligand superfamily member 13b, soluble form; tumor necrosis factor superfamily member 13b; tumor necrosis factor superfamily, member 13b; tumor necrosis factor-like protein ZTNF4; ZTNF4 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 17,29,31 Source/Isotype: Rabbit IgG