{"product_id":"cst-19984t","title":"CST,  19984T, AsCpf1\/Cas12a (Strain BV3L6) (E1U7C) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying AsCpf1 (Strain BV3L6). Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed\/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, AsCpf1\/Cas12a (Strain \u003ci\u003eBV3L6\u003c\/i\u003e) (E1U7C) Rabbit Monoclonal Antibody (CST #19984) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunofluorescence (Immunocytochemistry): 1:400\nFlow Cytometry (Fixed\/Permeabilized): 1:400\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 33209 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nAsCpf1\/Cas12a (Strain\nSpecies Reactivity: All Species Expected\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu822 of sp. Cpf1\/Cas12a (Strain ) protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCRISPR-Cas (clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins) are RNA-guided nuclease effectors that are utilized for precise genome editing in mammalian systems (1). Cpf1\/Cas12a (CRISPR from and ) proteins are members of the Class 2 CRISPR system (2). Class 2 CRISPR systems, such as the well characterized Cas9, rely on single-component effector proteins to mediate DNA interference (3). Cpf1\/Cas12a endonucleases, compared to Cas9 systems, have several unique features that increase the utility of CRISPR-based genome editing techniques: 1) Cpf1\/Cas12a-mediated cleavage relies on a single and short CRISPR RNA (crRNA) without the requirement of a trans-activating crRNA (tracrRNA), 2) Cpf1\/Cas12a utilizes T-Rich protospacer-adjacent motif (PAM) sequences rather than a G-Rich PAM, and 3) Cpf1\/Cas12a generates a staggered, rather than a blunt-ended, DNA double-stranded break (2). These features broaden the utility of using CRISPR-Cas systems for specific gene regulation and therapeutic applications. Several Cpf1\/Cas12a bacterial orthologs have been characterized for CRISPR-mediated mammalian genome editing (2,4). AsCpf1 (Strain )\/Cas12a is a Cpf1\/Cas12a enzyme derived from sp. (5,6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAsCpf1; Cpf1; CRISPR-associated endonuclease Cas12a; CRISPR-associated endonuclease Cpf1; HMPREF1246_0236\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: All\nSENSITIVITY: Transfected Only\nMW (kDa): 151\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800195616937,"sku":"19984T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-19984t","provider":"Iright","version":"1.0","type":"link"}