CST, 20030SF, CD2 (D6V9F) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying CD2. Validated for Western Blotting,IHC Leica Bond,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, CD2 (D6V9F) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #20030) is ready to ship. Product Usage Information This product is the carrier-free version of product #9987. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product #www.cellsignal.com/products/primary-antibodies/cd2-d6v9f-rabbit-mab/9987?site-search-type=Products&N=4294956287&Ntt=cd2&fromPage=plp">9987. Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity CD2 (D6V9F) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total CD2 protein. Non-specific staining was observed in skeletal muscle by immunohistochemistry. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala270 of human CD2 protein. Background CD2 is a transmembrane glycoprotein expressed early in thymocyte development and present on most circulating T cells (1,2). CD2 plays a role in T cell adhesion through binding to its ligand CD58 (LFA-3) (3). Stimulation of CD2 also leads to T cell activation and proliferation (2). T cells from mice deficient in both CD2 and CD28 have severe defects in T cell activation and function, while T cells deficient in either CD2 or CD28 are still capable of mounting a response, suggesting that CD2 and CD28 may have overlapping functions and may be able to compensate for each other (4). In addition, engagement of CD2 and CD58 was recently demonstrated to be the primary costimulatory signal in T cells that lack CD28 (5). CD2 expression also distinguishes a subset of plasmacytoid dendritic cells found in tumors and tonsils that express lysozyme, higher levels of IL-12 p40, and higher levels of CD80 (6). Alternate Names CD2; CD2 antigen (p50), sheep red blood cell receptor; CD2 molecule; Erythrocyte receptor; FLJ46032; LFA-2; LFA-3 receptor; lymphocyte-function antigen-2; Rosette receptor; SRBC; T-cell surface antigen CD2; T-cell surface antigen T11/Leu-5; T11 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 48-65 Source/Isotype: Rabbit IgG